SH-SY5Y neuroblasotoma ¼¼Æ÷¿¡¼ CoCl_2 À¯¹ß¼¼Æ÷°í»ç¿¡ ´ëÇÑ ¹ÌÅäÄܵ帮¾Æ ÀÇÁ¸ ¼¼Æ÷°í»ç ±âÀüÀÇ ¿ªÇÒ
Involvement of Mitochondria-dependent Apoptotic Pathway in CoCl_2-induced Apoptosis of SH-SY5Y Human Neuroblastoma Cells
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³ë±¤ÈÆ ( Roh Kwang-Hoon ) - Chonnam National University College of Densitry & Dental Science Resrarch Institute Department of Dental Physiology
±è¿øÀç ( Kim Won-Jae ) - Chonnam National University College of Densitry & Dental Science Resrarch Institute Department of Dental Physiology
KMID : 0988420020140040305
Abstract
Àú»ê¼Ò»óÅÂ(hypoxia) ¶Ç´Â ÇãÇ÷»óÅÂ(ischemia)¿¡¼´Â ¼¼Æ÷°í»ç (apoptosis)°¡ ÀϾÙ. ±×·¯³ª Àú»ê¼Ò»óÅ À¯¹ß¼¼Æ÷°í»ç ±âÀüÀ» ÀÏÀ¸Å°´Â ±âÀüÀº ¾ÆÁ÷ È®½ÇÈ÷ ±Ô¸íµÇ¾î ÀÖÁö ¾Ê´Ù. º» ¿¬±¸´Â ÀÎü SH-SY5Y neuroblastoma ¼¼Æ÷¿¡¼ Àú»ê¼Ò»óŸ¦ À¯¹ßÇÑ´Ù°í ¾Ë·ÁÁø CoC1_2À» Åõ¿©ÇÏ¿© ¼¼Æ÷°í»ç¸¦ ÀÏÀ¸Å°°í À̶§ ¹ÌÅäÄܵ帮¾Æ ÀÇÁ¸ ¼¼Æ÷°í»ç ±âÀüÀÇ ¿¬°ü¼ºÀ» ¹àÈ÷°íÀÚ ÇÏ¿´´Ù.
SH-SY5Y neuroblastoma ¼¼Æ÷¿¡ 2 ¥ìmote CoCl_2¸¦ Åõ¿©ÇÑ ÈÄ 30ºÐ, 1½Ã°£, 2½Ã°£, 4½Ã°£, 8½Ã°£, 24½Ã°£¿¡¼ ¼¼Æ÷¼ö°¡ ½Ã°£ÀÌ Áõ°¡ÇÔ¿¡ µû¶ó Á¡Â÷ °¨¼ÒÇÏ¿´´Ù. Internucleosomal DNA fragmentation (IDF) analysis °á°ú CoCl_2 Åõ¿©±º¿¡¼ ¼¼Æ÷°í»ç late stage¿¡¼ ¹ß»ýÇÏ´Â BNA fragmentationÀ» º¸¿´´Ù.
WesternºÐ¼® °á°úCoC1_2Åõ¿© ÈÄ ¹ÌÅäÄܵ帮¾Æ¿¡¼ ¼¼Æ÷Áú·Î À¯¸®µÈ´Ù°í ¾Ë·ÁÁø °í»ç±âÀü ¸Å°³ÀÎÀÚÀÎ cytochromec°¡ ½Ã°£ÀÌ °æ°úÇÔ¿¡ µû¶ó Áõ°¡ÇÏ¿´´Ù. ¶ÇÇÑ RT-PCR °á°ú CoC1_2 Åõ¿© ÈÄ cytochrome c¿¡ ÀÇÇÑ °í»ç±âÀüÀÇ down streamÀÎcaspase-9ÀÇ Àü±¸¹°ÁúÀÎ procaspase-9Àº°¨¼ÒÇÏ¿´´Ù. ´õ ³ª¾Æ°¡caspase-9 downstreamÀÎc aspase3ÀÇ ¹ßÇöÀº Áõ°¡ÇÏ¿´´Ù.
ÀÌ»óÀÇ ½ÇÇè°á°ú´Â SH-SY5Y neuroblastoma ¼¼Æ÷¿¡½Ã CoC1_2 À¯¹ß ¼¼Æ÷°í»ç ±âÀü¿¡ ¹ÌÅäÄܵ帮¾Æ ÀÇÁ¸ °í»ç±âÀüÀÌ °ü¿©ÇÔÀ» ½Ã»çÇÑ´Ù.
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